BLM antibody (C-Term)

Catalog No. ABIN2855003

The Rabbit Polyclonal anti-BLM antibody has been validated for WB, IF, IHC (p) and ICC. It is suitable to detect BLM in samples from Human and Mouse.

Quick Overview for BLM antibody (C-Term) (ABIN2855003)

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-BLM Antibody

Binding Specificity: C-Term

Characteristics: Rabbit Polyclonal antibody to BLM (Bloom syndrome, RecQ helicase-like)
BLM antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human BLM. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Jurkat , Raji , K562 , NCI-H929 , NIH-3T3 , JC , BCL-1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.35 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for BLM

Target: BLM (Bloom Syndrome RecQ Like Helicase (BLM))

Alternative Name: BLM

Background: The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination.

Cellular Localization: Nucleus

Molecular Weight: 159 kDa

Gene ID: 641

Pathways: DNA Damage Repair